Ziehl's carbol fuchsin stain being applied to slide over a heated water bath.
ANAT & PHYSGENERAL BIOCELL BIOMICRO 8wkMICRO 15wkINSTRUCTORS



Acid-fast Ziehl-Neelsen Staining of Bacteria​ - P2
Ziehl's carbol fuchsin stain being applied to slide over a heated water bath.
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Prokaryotic Cell, Mariana Ruiz
Steps of Acid Fast Staining Procedure
The protocol for staining acid-fast organisms is as follows (see photos below):

  • Place strip of blotting paper over the slide.

  • Place slide over a screened water bath. Then saturate blotting paper with primary stain Ziehl’s carbol fuschion.

  • Allow slide to sit over water bath for 3–5 minutes. Reapply stain if it begins to dry out.

  • Discard blotting paper. Rinse slide until water runs clear.

  • Flood slide with decolorizer, Acid Alcohol, for 10–15 seconds. Then rinse.

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How Acid-fast Stain Works
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​SPO VIDEO: How to Do an 
Acid-fast Bacterial Stain
  • ​Flood slide with counterstain, Crystal Violet, for one minute. Then rinse.


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