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Steps of Acid Fast Staining Procedure
The protocol for staining acid-fast organisms is as follows (see photos below):
Place strip of blotting paper over the slide.
Place slide over a screened water bath. Then saturate blotting paper with primary stain Ziehl’s carbol fuschion.
Allow slide to sit over water bath for 3–5 minutes. Reapply stain if it begins to dry out.
Discard blotting paper. Rinse slide until water runs clear.
Flood slide with decolorizer, Acid Alcohol, for 10–15 seconds. Then rinse.